DNA Contamination of RNA Vaccines
Pieces of DNA in two batches of Pfizer vaccine. These are the batches that were given out here in Columbia
the pieces of DNA are small and are likely to damage the human genome by integrating and
becoming permanent mutations (like shotgun pellets hitting a washboard). its important to look at
DNA taken from different body tissues of vaccinated people to see if this is happening and if it can
be causing any adverse events now or if there is a future cancer risk down the road. we should
sequence a few hundred people and find out if this DNA ever got into the human genome.
we used the sequences of all the little pieces of DNA in the vaccine to reconstruct the actual sequence of where it came from. It is the plasmid used in production of the mRNA (pBK-CMV modified to contain SPIKE gene). The DNA in the vaccine is a contaminate leftover from the process used in large scale production. This DNA was not present in the material used in the trials because the process of making the stuff was different (it did not use this plasmid DNA).
We have a pretty easy and cheap method to detect one of the pieces of plasmid DNA. It’s a PCR test similar to what we used to detect SARS-COV2 during the pandemic (the saliva test).
There are about 2 billion copies of the fragment containing the origin of replication, and from nanopore sequence analysis, there is probably 50-100 times that many pieces of plasmid DNA derived from the entire vector.
This means each shot has about 200 billion pieces of plasmid DNA encapsulated in the lipid nanoparticle.
This is a bad idea.
Philips Buckhaults, Ph.D. Professor of Cancer Molecular Genetics University of South Carolina
Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose
Purifying the mRNA from the LNPs
Library Construction for Sequencing
Raw Illumina Reads RNA-seq
Kevin McKernan, Yvonne Helbert, Liam T. Kane, Stephen McLaughlin